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phal  (Vector Laboratories)


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    Structured Review

    Vector Laboratories phal
    Phal, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 110 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/AS-2224-1/Anti-Phaseolus+Vulgaris+Agglutinin+(E%2BL)%2C+Unconjugated%2C+Made+in+Goat/bio_rxiv__2025__01__18__633747-96-11-16
    Average 93 stars, based on 110 article reviews
    phal - by Bioz Stars, 2026-11
    93/100 stars

    Images

    Related Articles

    Injection:

    Article Title: Organization of projections from the entorhinal cortex to the hippocampal formation of the Egyptian fruit bat Rousettus aegyptiacus.
    Article Snippet: Funding information H2020 European Research Council, Grant/Award Number: ERC-StG NEUROBAT; Kavli Foundation; Kimmel Award for Innovative Investigation; Norwegian Research Council, Grant/Award Number: 223262 Abstract The hippocampal formation and entorhinal cortex are crucially involved in learning and memory as well as in spatial navigation.. The conservation of these structures across the entire mammalian lineage demonstrates their importance.. Information on a diverse set of spatially tuned neurons has become available, but we only have a rudimentary understanding of how anatomical network structure affects functional tuning.

    Staining:

    Article Title: Organization of projections from the entorhinal cortex to the hippocampal formation of the Egyptian fruit bat Rousettus aegyptiacus.
    Article Snippet: Funding information H2020 European Research Council, Grant/Award Number: ERC-StG NEUROBAT; Kavli Foundation; Kimmel Award for Innovative Investigation; Norwegian Research Council, Grant/Award Number: 223262 Abstract The hippocampal formation and entorhinal cortex are crucially involved in learning and memory as well as in spatial navigation.. The conservation of these structures across the entire mammalian lineage demonstrates their importance.. Information on a diverse set of spatially tuned neurons has become available, but we only have a rudimentary understanding of how anatomical network structure affects functional tuning.

    other:

    Article Title: The efferent connections of the orbitofrontal, posterior parietal, and insular cortex of the rat brain.
    Article Snippet: MRXS or CZI images provides the user with full access to explore the data in viewer tools (Pannoramic Viewer, 3DHISTECH Ltd, Budapest, Hungary, or ZEN 2.6 Blue edition, Carl Zeiss Microscopy, Jena, Germany, respectively) or to re-export images if needed, e.g., if new parameters or Type Name Target antigen Source ID Dilution (OFC) Dilution (PPC/ IC) Primary antibody goat anti-PHA-l, polyclonal PHA-l Vector Laboratories Cat No.AS-2224 W0131; RRID:AB_10000080 1:2000 1:1000 Secondary antibody Donkey anti-Goat IgG (H + L) CrossAdsorbed Secondary Antibody, Alexa FluorTM 488 and 546 conjugate goat anti-PHA-l Sigma-Aldrich, St. Louis, MO AlexaFluor488: RRID:AB_142672, Cat No. A11055; AlexaFluor546: RRID:AB_142628, Cat No. A11056 1:100 1:200 Tertiary antibody goat peroxidase-anti-peroxidase donkey anti-goat IgG Sigma-Aldrich, St. Louis, MO Product No. P1901 1:200 1:800 Fluorescent dye Streptavidin, Alexa FluorTM 488 and 546 conjugate BDA Invitrogen, Molecular Probes AlexaFluor488: Cat No. S11223; AlexaFluor546: Cat No. S11225 NA 1:200 Chromogenic substrate 3,3′-Diaminobenzidine tetrahydrochloride (DAB) NA Sigma-Aldrich, St. Louis, MO Cat No. D5905 0.067% 0.067% Table 2.

    Article Title: The efferent connections of the orbitofrontal, posterior parietal, and insular cortex of the rat brain
    Article Snippet: Primary antibody , goat anti-PHA-l, polyclonal , PHA-l , Vector Laboratories , Cat No.AS-2224 W0131; RRID:AB_10000080 , 1:2000 , 1:1000.

    Immunohistochemistry:

    Article Title: Method for producing disease modeling non-human animal, disease modeling non-human animal, and method for screening drug and method for determining risk of disease using the same
    Article Snippet: The following antibodies were used for the flow cytometry analysis: FITC-conjugated anti-CD19 antibody (eBioscience), anti-CD11b antibody (eBioscience), anti-CD44 antibody (eBioscience), anti-CD4 antibody (eBioscience), PE-conjugated anti-CD44 antibody (eBioscience), anti-TCR antibody (eBioscience), PE-Cy7-conjugated anti-CD90.2 antibody (eBioscience), APC-conjugated anti-CD4 antibody (BioLegend), anti-I-A/I-E antibody (BioLegend), biotin-conjugated anti-CD11b antibody (eBioscience), anti-CD19 antibody (eBioscience), anti-NK1.1 antibody (eBioscience), anti-CD11c antibody (eBioscience), and anti-TCR antibody (eBioscience). .. The following antibodies were used for immunohistochemistry: FITC-conjugated anti-I-A/I-E antibody (BioLegend), anti-dopamine transporter antibody (Abcam plc.), anti-noradrenaline transporter antibody (Abcam plc.), anti-phosphorylated CREB antibody (Cell Signaling Technology, Inc.), anti-tyrosine hydroxylase antibody (Abcam plc.), anti-phosphorylated c-Fos (Ser32) (Cell Signaling Technology, Inc.), control rabbit IgG (DAlE) (Cell Signaling Technology, Inc.), biotin-conjugated anti-CD4 antibody (BioLegend), anti-CD11b antibody (eBioscience), anti-I-A/I-E antibody (BioLegend), anti-Phaseolus vulgaris agglutinin antibody (VECTOR, Inc.), anti-CD31 antibody (Abcam plc.), anti-activated caspase3 antibody (Cell Signaling Technology, Inc.), anti-CHT1 antibody (produced by Department of Anatomy and Embryology, Graduate School of Medicine, Hokkaido University), Alexa Fluor 546 donkey anti-goat IgG antibody (H+L), Alexa Fluor 488 goat anti-rabbit IgG antibody (H+L), Alexa Fluor 546 goat anti-rabbit IgG antibody (H+L), Alexa Fluor 647 goat anti-rabbit IgG antibody (H+L), Alexa Fluor 647 goat anti-chicken IgG antibody (Invitrogen), and streptavidin Alexa Fluor 546 conjugate (Invitrogen). .. In in vivo neutralization tests, the following antibodies were used: Anti-mouse IL-17 antibody and anti-CCL5 antibody (both from R&D Systems, Inc.), anti-IFN-γ antibody (purified according to Ueda et al. (2006), Int.

    Control:

    Article Title: Method for producing disease modeling non-human animal, disease modeling non-human animal, and method for screening drug and method for determining risk of disease using the same
    Article Snippet: The following antibodies were used for the flow cytometry analysis: FITC-conjugated anti-CD19 antibody (eBioscience), anti-CD11b antibody (eBioscience), anti-CD44 antibody (eBioscience), anti-CD4 antibody (eBioscience), PE-conjugated anti-CD44 antibody (eBioscience), anti-TCR antibody (eBioscience), PE-Cy7-conjugated anti-CD90.2 antibody (eBioscience), APC-conjugated anti-CD4 antibody (BioLegend), anti-I-A/I-E antibody (BioLegend), biotin-conjugated anti-CD11b antibody (eBioscience), anti-CD19 antibody (eBioscience), anti-NK1.1 antibody (eBioscience), anti-CD11c antibody (eBioscience), and anti-TCR antibody (eBioscience). .. The following antibodies were used for immunohistochemistry: FITC-conjugated anti-I-A/I-E antibody (BioLegend), anti-dopamine transporter antibody (Abcam plc.), anti-noradrenaline transporter antibody (Abcam plc.), anti-phosphorylated CREB antibody (Cell Signaling Technology, Inc.), anti-tyrosine hydroxylase antibody (Abcam plc.), anti-phosphorylated c-Fos (Ser32) (Cell Signaling Technology, Inc.), control rabbit IgG (DAlE) (Cell Signaling Technology, Inc.), biotin-conjugated anti-CD4 antibody (BioLegend), anti-CD11b antibody (eBioscience), anti-I-A/I-E antibody (BioLegend), anti-Phaseolus vulgaris agglutinin antibody (VECTOR, Inc.), anti-CD31 antibody (Abcam plc.), anti-activated caspase3 antibody (Cell Signaling Technology, Inc.), anti-CHT1 antibody (produced by Department of Anatomy and Embryology, Graduate School of Medicine, Hokkaido University), Alexa Fluor 546 donkey anti-goat IgG antibody (H+L), Alexa Fluor 488 goat anti-rabbit IgG antibody (H+L), Alexa Fluor 546 goat anti-rabbit IgG antibody (H+L), Alexa Fluor 647 goat anti-rabbit IgG antibody (H+L), Alexa Fluor 647 goat anti-chicken IgG antibody (Invitrogen), and streptavidin Alexa Fluor 546 conjugate (Invitrogen). .. In in vivo neutralization tests, the following antibodies were used: Anti-mouse IL-17 antibody and anti-CCL5 antibody (both from R&D Systems, Inc.), anti-IFN-γ antibody (purified according to Ueda et al. (2006), Int.

    Produced:

    Article Title: Method for producing disease modeling non-human animal, disease modeling non-human animal, and method for screening drug and method for determining risk of disease using the same
    Article Snippet: The following antibodies were used for the flow cytometry analysis: FITC-conjugated anti-CD19 antibody (eBioscience), anti-CD11b antibody (eBioscience), anti-CD44 antibody (eBioscience), anti-CD4 antibody (eBioscience), PE-conjugated anti-CD44 antibody (eBioscience), anti-TCR antibody (eBioscience), PE-Cy7-conjugated anti-CD90.2 antibody (eBioscience), APC-conjugated anti-CD4 antibody (BioLegend), anti-I-A/I-E antibody (BioLegend), biotin-conjugated anti-CD11b antibody (eBioscience), anti-CD19 antibody (eBioscience), anti-NK1.1 antibody (eBioscience), anti-CD11c antibody (eBioscience), and anti-TCR antibody (eBioscience). .. The following antibodies were used for immunohistochemistry: FITC-conjugated anti-I-A/I-E antibody (BioLegend), anti-dopamine transporter antibody (Abcam plc.), anti-noradrenaline transporter antibody (Abcam plc.), anti-phosphorylated CREB antibody (Cell Signaling Technology, Inc.), anti-tyrosine hydroxylase antibody (Abcam plc.), anti-phosphorylated c-Fos (Ser32) (Cell Signaling Technology, Inc.), control rabbit IgG (DAlE) (Cell Signaling Technology, Inc.), biotin-conjugated anti-CD4 antibody (BioLegend), anti-CD11b antibody (eBioscience), anti-I-A/I-E antibody (BioLegend), anti-Phaseolus vulgaris agglutinin antibody (VECTOR, Inc.), anti-CD31 antibody (Abcam plc.), anti-activated caspase3 antibody (Cell Signaling Technology, Inc.), anti-CHT1 antibody (produced by Department of Anatomy and Embryology, Graduate School of Medicine, Hokkaido University), Alexa Fluor 546 donkey anti-goat IgG antibody (H+L), Alexa Fluor 488 goat anti-rabbit IgG antibody (H+L), Alexa Fluor 546 goat anti-rabbit IgG antibody (H+L), Alexa Fluor 647 goat anti-rabbit IgG antibody (H+L), Alexa Fluor 647 goat anti-chicken IgG antibody (Invitrogen), and streptavidin Alexa Fluor 546 conjugate (Invitrogen). .. In in vivo neutralization tests, the following antibodies were used: Anti-mouse IL-17 antibody and anti-CCL5 antibody (both from R&D Systems, Inc.), anti-IFN-γ antibody (purified according to Ueda et al. (2006), Int.

    Incubation:

    Article Title: Projections from the five divisions of the orbital cortex to the thalamus in the rat.
    Article Snippet: Funding information National Institutes of Health, Grant/Award Numbers: NS108259, NS119847 Abstract The orbital cortex (ORB) of the rat consists of five divisions: the medial (MO), ventral (VO), ventrolateral (VLO), lateral (LO), and dorsolateral (DLO) orbital cortices.. No previous report has comprehensively examined and compared projections from each division of the ORB to the thalamus.. Using the anterograde anatomical tracer, Phaseolus vulgaris leucoagglutinin, we describe the efferent projections from the five divisions of the ORB to the thalamus in the rat.

    Article Title: Elaborating the connections of a closed-loop forebrain circuit in the rat: Circumscribed evidence for novel topography within a cortico-striato-pallidal triple descending projection, with thalamic feedback, to the anterior lateral hypothalamic area
    Article Snippet: After another 5 × 5 wash, we incubated tissues for 2 h in a blocking solution containing 2% (v/v) normal donkey serum (EMD Millipore; Catalog #S30) and 0.1% Triton X-100 (Sigma-Aldrich; Catalog #T8532). .. We then incubated tissues in primary antiserum containing antibodies raised against PHAL (host: rabbit; dilution: 1:4,000; Vector Labs, Cat # AS-2224; RRID: AB_2313686) or CTB (host: goat; dilution: 1:10,000; List Biological Cat # 703; RRID: AB_10013220) in blocking solution for 48 h in 4°C. .. After a 5 × 5 TBS wash, we incubated the sections in secondary antiserum with biotinylated antibodies raised against rabbit (host: donkey; dilution: 1:1,000; Jackson ImmunoResearch Labs, Cat# 711-065-152; RRID: AB_2340593) or goat (host: donkey; dilution: 1:1,000; Jackson ImmunoResearch Labs, Cat# 705-065-147; RRID: AB_2340397) for 5 h followed by another 5 × 5 TBS wash. Next, we amplified signal via formation of avidin-biotin-horseradish peroxidase complexes (Vectastain ABC HRP Kit, Vector, 45 μl reagent A and B per 10 ml) in 0.05 M TBS containing 0.1% Triton X-100 (v/v) for 1 h. We rinsed tissues and developed them in 0.05% 3, 3’-diaminobenzidine (DAB) (Sigma-Aldrich) mixed with 0.015% H 2 O 2 in 0.05 M TBS for 10–20 min for PHAL.

    Concentration Assay:

    Article Title: Projections from the five divisions of the orbital cortex to the thalamus in the rat.
    Article Snippet: Funding information National Institutes of Health, Grant/Award Numbers: NS108259, NS119847 Abstract The orbital cortex (ORB) of the rat consists of five divisions: the medial (MO), ventral (VO), ventrolateral (VLO), lateral (LO), and dorsolateral (DLO) orbital cortices.. No previous report has comprehensively examined and compared projections from each division of the ORB to the thalamus.. Using the anterograde anatomical tracer, Phaseolus vulgaris leucoagglutinin, we describe the efferent projections from the five divisions of the ORB to the thalamus in the rat.

    Blocking Assay:

    Article Title: Elaborating the connections of a closed-loop forebrain circuit in the rat: Circumscribed evidence for novel topography within a cortico-striato-pallidal triple descending projection, with thalamic feedback, to the anterior lateral hypothalamic area
    Article Snippet: After another 5 × 5 wash, we incubated tissues for 2 h in a blocking solution containing 2% (v/v) normal donkey serum (EMD Millipore; Catalog #S30) and 0.1% Triton X-100 (Sigma-Aldrich; Catalog #T8532). .. We then incubated tissues in primary antiserum containing antibodies raised against PHAL (host: rabbit; dilution: 1:4,000; Vector Labs, Cat # AS-2224; RRID: AB_2313686) or CTB (host: goat; dilution: 1:10,000; List Biological Cat # 703; RRID: AB_10013220) in blocking solution for 48 h in 4°C. .. After a 5 × 5 TBS wash, we incubated the sections in secondary antiserum with biotinylated antibodies raised against rabbit (host: donkey; dilution: 1:1,000; Jackson ImmunoResearch Labs, Cat# 711-065-152; RRID: AB_2340593) or goat (host: donkey; dilution: 1:1,000; Jackson ImmunoResearch Labs, Cat# 705-065-147; RRID: AB_2340397) for 5 h followed by another 5 × 5 TBS wash. Next, we amplified signal via formation of avidin-biotin-horseradish peroxidase complexes (Vectastain ABC HRP Kit, Vector, 45 μl reagent A and B per 10 ml) in 0.05 M TBS containing 0.1% Triton X-100 (v/v) for 1 h. We rinsed tissues and developed them in 0.05% 3, 3’-diaminobenzidine (DAB) (Sigma-Aldrich) mixed with 0.015% H 2 O 2 in 0.05 M TBS for 10–20 min for PHAL.



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    Image Search Results


    Antibodies and visualising agents.

    Journal: Scientific Data

    Article Title: The efferent connections of the orbitofrontal, posterior parietal, and insular cortex of the rat brain

    doi: 10.1038/s41597-023-02527-y

    Figure Lengend Snippet: Antibodies and visualising agents.

    Article Snippet: Primary antibody , goat anti-PHA-l, polyclonal , PHA-l , Vector Laboratories , Cat No.AS-2224 W0131; RRID:AB_10000080 , 1:2000 , 1:1000.

    Techniques: Plasmid Preparation

    Antibodies and visualising agents.

    Journal: Scientific Data

    Article Title: The efferent connections of the orbitofrontal, posterior parietal, and insular cortex of the rat brain

    doi: 10.1038/s41597-023-02527-y

    Figure Lengend Snippet: Antibodies and visualising agents.

    Article Snippet: Primary antibody , goat anti-PHA-l, polyclonal , PHA-l , Vector Laboratories , Cat No.AS-2224 W0131; RRID:AB_10000080 , 1:2000 , 1:1000.

    Techniques: Plasmid Preparation